NaOH-Fenton试剂预处理桑木的纤维素酶分段酶水解研究

doi:10.3969/j.issn.1673-5854.2016.04.006

Abstract

Abstract: During the multi-stage enzymatic hydrolysis of lignocellulosic materials, the product inhibitions were reduced by the removal of end products at each stage to enhance the hydrolysis efficiency and shorten the hydrolysis time with NaOH-Fenton pretreated mulberry as raw material. During the three-stage (8+8+8h) hydrolysis, as the enzymatic dosage was 15FPIU/g(calculated by the mass of cellulose, similarly here in after), enzymatic average reaction rate increased from 1.25 to 2.21g/(L·h) at the first stage, and after the second stage, it reached 1.54g/(L·h) and increased rapidly by 73% comparing with that of unsegmented hydrolysis.When enzymatic dosage was 40FPIU/g, the three-stage (8+8+8h) hydrolysis could reach 88.08%.Multi-stage hydrolysis made full use of the absorbed cellulase on substrate.The classical Langmuir adsorption isotherm was used to fit the cellulase enzyme adsorption on substrates.The adsorption capacity of cellulase on NaOH-Fenton pretreated mulberry was studied with the maximum adsorption capacity of 8.08mg/g substrate.The adsorbed sites were enhanced in the pretreatment process.

Key words: cellulase, enzymatic hydrolysis, enzymatic reaction rate, cellulose adsorption

CLC Number:

TQ35

WANG Yan-yun, WU Kai, LEI Fu-hou, YANG Jing. Process Resrarch of Multi-stage Enzymatic Hydrolysis of NaOH-Fenton Pretreated Mulberry Wood[J]. bce, 2016, 50(4): 31-36.

References

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Process Resrarch of Multi-stage Enzymatic Hydrolysis of NaOH-Fenton Pretreated Mulberry Wood

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