Abstract: The optimum reaction conditions of alkaline-Fenton pretreatment of bamboo were obtained through the response surface methodology. The results showed that the optimum conditions of Fenton reaction were alkaline treated bamboo 1 g, 30% H₂O₂3.4 mL, concentration of Fe²⁺15.8 mmol/L, and reaction time 12 h. The 72 h enzymatic hydrolysis yield was 49.98%. The sample pretreated by 2% NaOH-Fenton had higher cellulose content and lower hemicellulose and lignin content compared with the materials and sample pretreated by 2% NaOH. The 72 h enzymatic hydrolysis yield was 48.24% and increased by 47.79% and 37.44%, respectively. The 72 h enzymatic hydrolysis yield was 76.64% when the dosage of the cellulase and the β-glucosidase were 32 FPIU/g and 16 IU/g, respectively. This was 22.80% higher than that with the single use of the cellulase 32 FPIU/g.

Key words: response surface methodology, Fenton reaction, enzymatic hydrolysis yield