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bce ›› 2015, Vol. 49 ›› Issue (4): 25-30.doi: 10.3969/j.issn.1673-5854.2015.04.005

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Fermentation Optimization of Fuel Ethanol Producing by Simultaneous Liquefaction,Saccharification and Fermentation from Acorn Flour

ZHANG Ning, JIANG Jian-chun, YANG Jing, WEI Min, ZHAO Jian, CHEN Shui-gen   

  1. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA; Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China
  • Received:2015-03-12 Online:2015-07-30 Published:2015-10-12

Abstract: The fuel ethanol was produced from acron flour by simultaneous liquefaction,saccharification and fermentation (SLSF).The optimum conditions of fermentation by SLSF technology were determined through single factor experiments and orthogonal experiments.The results showed that SLSF technology was suitable for the ethanol fermentation from acorn flour.The optimum conditions were 20 g acorn flour removed of tannin,the ratio of materials to water 1:3,amylase 100 U/g,glucoamylase 3 750 U/g and active dry yeast 1.50%.The ethanol concentration of fermentation broth reached 106.5 g/L after static fermentation for 120 h at 30 ℃,and the conversion rate of acorn starch into ethanol reached 89.36%.The ethanol concentration of fermentation liquor by simultaneous liquefaction,saccharification and fermentation was comparable to the ethanol concentration fermented by corn and other food crops.

Key words: acorn flour, simultaneous liquefaction,saccharification and fermentation, fuel ethanol

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