响应曲面法优化小麦秸秆纤维素酶水解条件

doi:10.3969/j.issn.1673-5854.2015.02.008

Abstract

Abstract: With an aim to optimize the conditions of enzymatic hydrolysis of wheat straw,the parameters including solid content,enzyme loading,temperature,pH,and hydrolysis time were investigated by means of response surface methodology(RSM) with the reducing sugar(RS) yield as response value.The results showed that all the parameters had significant effects(p<0.05) on the reducing sugar yield.A well fitted regression equation with R² value of 0.946 9,p<0.05,coefficient variability(C_v) value of 4.37%,and adequate precision value of 26.396 indicated that the developed model was significant and could be used to navigate the designed space and predict the response.The predicted optimum conditions for enzymatic hydrolysis were solid content of 8.0%,enzyme loading of 35 FPU/g,temperature of 50 ℃,pH of 5.4,and hydrolysis time of 96 h.The experimental results showed that under the optimum conditions the corresponding RS yield was 60.73% with glucose and xylose concentrations of 31.84 and 16.74 g/L,respectively.Further,the results obtained in this research showed a high RS yield with high initial solid content,which would significantly improve the ethanol concentration,reduce the subsequently distillation costs,and improve the commercial potential of the lignocellulosic bioethanol production.

Key words: wheat straw, enzymatic hydrolysis, response surface methodology, reducing sugar

CLC Number:

ZHANG Wei, ZHANG Qi, RUAN Xin-yi, WANG Xin-ze, KONG Hai-nan, LIN Yan. Optimization of Enzymatic Hydrolysis of Wheat Straw by Using Response Surface Methodology[J]. bce, 2015, 49(2): 39-46,52.

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Optimization of Enzymatic Hydrolysis of Wheat Straw by Using Response Surface Methodology

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