高效液相色谱-内标法测定焦性没食子酸含量

doi:10.3969/j.issn.1673-5854.2015.02.003

bce ›› 2015, Vol. 49 ›› Issue (2): 12-16.doi: 10.3969/j.issn.1673-5854.2015.02.003

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Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method

ZHANG Liang-liang, WANG Yong-mei, XU Man, CHEN Jia-hong

Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.of Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

Received:2014-07-16 Online:2015-03-30 Published:2015-04-07

Abstract

Abstract: A rapid,specific,and accurate HPLC method was developed and validated for the assay of pyrogallol.The assay involved a gradient elution of pyrogallol with a Thermo Hypersil C₁₈ column (250 mm×4.6 mm,5 μm) using mobile phase composition of methanol and water (0.1% TFA) at a flow rate of 1.0 mL/min.UV detection was performed at 266 nm.Ellagic acid was used as an internal standard.The retention time of pyrogallol and ellagic acid was 4.33 and 19.25 min,respectively.The linearity range of pyrogallol concentration was from 62.5 to 2 000 mg/L.The fitting equation of calibration curve was y=0.000 138 66x+0.001 92 (R=0.999 7) and a mean recovery of 103.6% was found.The proposed method was applied for the quantitative determination of pyrogallol in pyrogallol products.

Key words: pyrogallol, ellagic acid, RP-HPLC, internal standard method

CLC Number:

TQ35

ZHANG Liang-liang, WANG Yong-mei, XU Man, CHEN Jia-hong. Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method[J]. bce, 2015, 49(2): 12-16.

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Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method

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