多元复合酶降解黄姜薯蓣皂苷元的工艺研究

doi:10.3969/j.issn.1673-5854.2014.04.005

Abstract

Abstract: This paper focused on the impact factors of enzymatic hydrolysis by applying multivariate composite enzyme in the process. The results indicated that the quaternary multivariate composite enzyme, i.e.alkaline pectinase+tannase+hemicellulase+β-glycosidase, had the best degradation effect. The highest yield of diosgenin was 4.34%, when the condition was 1.5% compound enzyme of turmeric under 50℃, pH value 6.5, solid-liquid ratio of 1:4 and ethanol as extraction solvent for 10 h. Compared with the traditional acid hydrolysis which yield was only 1.61%,application of multivariate composite enzyme hydrolysis can improve the highest yield of diosgenin to 1.69 times.

Key words: yellow ginger, diosgenin, multivariate composite enzyme, enzymatic hydrolysis

CLC Number:

TQ35

LI Wen-jun, WANG Cheng-zhang, ZHANG Shui-jing, LU Li. Application of Multivariate Composite Enzymatic Hydrolysis in Extraction of the Diosgenin from Yellow Ginger[J]. bce, 2014, 48(4): 23-27.

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Application of Multivariate Composite Enzymatic Hydrolysis in Extraction of the Diosgenin from Yellow Ginger

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