两种木质素对纤维素酶水解的影响机制

doi:10.3969/j.issn.1673-5854.2018.02.006

生物质化学工程 ›› 2018, Vol. 52 ›› Issue (2): 29-34.doi: 10.3969/j.issn.1673-5854.2018.02.006

两种木质素对纤维素酶水解的影响机制

吴凯¹, 应文俊¹, 郑志锋², 史正军¹, 杨海艳¹, 杨静¹

1. 西南林业大学化学工程学院, 云南昆明 650224;
2. 云南省生物质高效利用工程实验室, 西南林业大学, 云南昆明 650224

收稿日期:2017-01-12 出版日期:2018-03-30 发布日期:2018-04-03
通讯作者: 杨静,副教授,博士,硕士生导师,主要从事木质纤维素的生物降解与转化的研究;E-mail:kmjingyang@163.com。 E-mail:kmjingyang@163.com
作者简介:吴凯(1993-),男,云南景洪人,硕士生,研究方向为生物质能源;E-mail:526602613@qq.com
基金资助:
云南省农业基础研究联合专项项目（2017FG001-025）；云南省教育厅科学研究基金项目（2017YJS092）；国家自然科学基金资助项目（3176030250）

Effect of Two Kinds of Lignin on Enzymatic Hydrolysis of Cellulose

WU Kai¹, YING Wenjun¹, ZHENG Zhifeng², SHI Zhengjun¹, YANG Haiyan¹, YANG Jing¹

1. College of Chemical Engineering, Southwest Forestry University, Kunming 650224, China;
2. Laboratory for Highly-efficient Utilization of Biomass, Yunnan Province, Southwest Forestry University, Kunming 650224, China

Received:2017-01-12 Online:2018-03-30 Published:2018-04-03

摘要/Abstract

摘要： 为探究木质素对纤维素酶水解效率的影响，将苦竹中提取的乙醇木质素（EOL-B）和磨木木质素（MWL-B）作为模型物添加到微晶纤维素中进行酶吸附和水解。结果表明：添加8 g/L MWL-B使得反应72 h的葡萄糖得率从51.34%降低到46.06%；添加8 g/L EOL-B使得反应72 h葡萄糖得率从51.34%增加到61.06%。与MWL-B相比，EOL-B与纤维素酶蛋白之间亲和力和结合力较低，故纤维素酶在EOL-B上的非特异吸附更少。FT-IR和¹³C NMR分析表明：经乙醇处理后，木质素分子中C-C凝缩单元减少，β-O-4'键断裂，导致木质素分子的亲水性增加，阻断了与纤维素酶蛋白疏水性氨基酸的结合，对纤维素酶蛋白吸附量减少，从而使得纤维底物周围的酶蛋白浓度增加，水解率提高。

关键词: 苦竹, 乙醇木质素, 磨木木质素, 酶水解, 亲水性基团

Abstract: In order to investigate the effect of lignin on the enzymatic hydrolysis of cellulose, the ethanol organosolv lignins (EOL-B) and milled wood lignin(MWL-B) were extracted from bitter bamboo and added to the microcrystalline cellulose for enzymatic digestibility and adsorption. The results showed that the addition of MWL-B(8 g/L) decreased the 72 h hydrolysis yields of glucose from 51.34% to 46.06%. But the addition of EOL-B(8 g/L) significantly improved the 72 h hydrolysis yields of glucose from 51.34% to 61.06%. Langmuir adsorption isotherms showed that EOL-B had lower binding ability and maximum adsorption capacity to the cellulase enzymes than MWL-B, which led to less non-productive binding. And FT-IR and ¹³C NMR spectra showed that the condensed structure of C-C of lignin reduced and the β-O-4' linkages cracked in the ethanol organosolv process, which resulted in increasing the hydrophilicity of lignin, blocking the combination of lignin and the hydrophobic amino acid of cellulose, decreasing the adsorption of cellulose, increasing the cellulose concentration around the cellulose substrate and promoting the effect of enzymatic hydrolysis.

Key words: bitter bamboo, ethanol organosolv lignins, milled wood lignin, enzymatic hydrolysis, hydrophilic group

中图分类号:

TQ35

吴凯, 应文俊, 郑志锋, 史正军, 杨海艳, 杨静. 两种木质素对纤维素酶水解的影响机制[J]. 生物质化学工程, 2018, 52(2): 29-34.

WU Kai, YING Wenjun, ZHENG Zhifeng, SHI Zhengjun, YANG Haiyan, YANG Jing. Effect of Two Kinds of Lignin on Enzymatic Hydrolysis of Cellulose[J]. bce, 2018, 52(2): 29-34.

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两种木质素对纤维素酶水解的影响机制

Effect of Two Kinds of Lignin on Enzymatic Hydrolysis of Cellulose

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