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生物质化学工程 ›› 2018, Vol. 52 ›› Issue (1): 10-16.doi: 10.3969/j.issn.1673-5854.2018.01.002

• 研究报告 • 上一篇    下一篇

金银忍冬提取物的挥发性成分及抑菌活性分析

高欣妍1, 王海英1, 刘志明2, 段晓玲1, 冯馨慧1   

  1. 1. 东北林业大学 林学院, 黑龙江 哈尔滨 150040;
    2. 东北林业大学 材料科学与工程学院, 黑龙江 哈尔滨 150040
  • 收稿日期:2016-12-05 出版日期:2018-01-30 发布日期:2018-02-06
  • 通讯作者: 王海英,副教授,博士,硕士生导师,主要从事植物资源化学以及木醋液、精油、纤维素等植物资源开发利用研究;E-mail:haiyingwang908@126.com。 E-mail:haiyingwang908@126.com
  • 作者简介:高欣妍(1994-),女,黑龙江哈尔滨人,硕士生,主要从事植物资源开发利用研究
  • 基金资助:
    黑龙江省自然科学基金资助项目(C201411);技术开发项目企业横向课题(07043215003)

Analysis of Volatile Components and Antibacterial Activity of Lonicera maackii(Rupr.) Maxim. Extracts

GAO Xinyan1, WANG Haiying1, LIU Zhiming2, DUAN Xiaoling1, FENG Xinhui1   

  1. 1. College of Forestry, Northeast Forestry University, Harbin 150040, China;
    2. College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China
  • Received:2016-12-05 Online:2018-01-30 Published:2018-02-06

摘要: 采用索氏提取法,以正己烷为溶剂,提取金银忍冬鲜叶和鲜花中的活性物质,通过气相色谱-质谱(GC-MS)法分析其挥发性化学成分并通过抑菌活性实验分析其抑菌活性。分析结果表明:金银忍冬鲜叶提取物中GC含量最高的化合物是(-)-异长叶醇(22.63%),其次是2,7,10-三甲基-十二烷(10.48%)。金银忍冬鲜花提取物中GC含量最高的化合物是2,7,10-三甲基-十二烷(14.16%),其次是邻苯二甲酸二丁酯(9.60%)。金银忍冬鲜叶提取物质量浓度为0.19 g/mL时,对金黄色葡萄球菌的抑制作用最明显,抑菌圈直径达到13.81±1.48 mm;金银忍冬鲜花提取物在质量浓度0.027 5~0.22 g/mL范围内,对大肠杆菌有较好的抑制作用。根据半数抑制浓度比较提取物对3种供试菌种的抑制能力,结果显示金银忍冬鲜叶及其鲜花提取物对3种实验菌的抑制效果顺序均为金黄色葡萄球菌>大肠杆菌>枯草芽孢杆菌。

关键词: 金银忍冬提取物, GC-MS, 抑菌活性

Abstract: Active substance of Lonicera maackii(Rupr.) Maxim. fresh leaves and fresh flowers were respectively extracted by Soxhlet extraction method with hexane as solvent. The volatile components were analyzed by gas chromatography-mass spectrometry(GC-MS),and their antibacterial activities were analyzed by antibacterial activity experiment. The results showed that the highest GC content compound was (-)-isolongifolol(22.63%), the second was 2,7,10-trimethyl-dodecane(10.48 %) in the fresh leaves extracts of Lonicera maackii. The highest GC content compound was 2,7,10-trimethyl-dodecane(14.16 %), and the second was the dibutyl phthalate(9.60 %) in the fresh flowers extracts of Lonicera maackii. When the mass concentration of fresh leaves extracts of Lonicera maackii was 0.19 g/mL, the inhibitory effect on Staphylococcus aureus was the highest with the antibacterial circle diameter of 13.81±1.48 mm. Lonicera maackii(Rupr.) Maxim. fresh flowers extract had good inhibition effect on Escherichia coli in the mass concentration of 0.027 5-0.22 g/mL range. According to the half inhibitory concentration of Lonicera maackii(Rupr.) Maxim. extract on three kinds of test strains, the results showed that Lonicera maackii(Rupr.) Maxim. fresh leaves extract and its flower extract of three kinds of experimental bacteria followed the inhibitory sequence of Staphylococcus aureus > Escherichia coli > Bacillus subtilis.

Key words: Lonicera maackii extracts, gas chromatography-mass spectrometry (GC-MS), antibacterial activity

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