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生物质化学工程 ›› 2017, Vol. 51 ›› Issue (6): 43-47.doi: 10.3969/j.issn.1673-5854.2017.06.008

• 研究报告 • 上一篇    下一篇

超声波辅助提取银杏落叶中多糖的工艺初探

郭香琴1,2, 蔡娜1, 杨善彬1,2, 周石洋1,2, 陈意心1, 柯道瑶1   

  1. 1. 重庆师范大学 化学学院, 重庆 401331;
    2. 重庆师范大学 活性物质生物技术教育部工程研究中心, 重庆 401331
  • 收稿日期:2016-10-16 出版日期:2017-11-30 发布日期:2017-12-08
  • 通讯作者: 杨善彬,博士,教授,硕士生导师,研究方向为天然产物化学、制药工程;E-mail:shanbiny@126.com。 E-mail:shanbiny@126.com
  • 作者简介:郭香琴(1991-),女,重庆人,硕士生,研究方向为化学工程;E-mail:1072271995@qq.com
  • 基金资助:
    活性物质生物技术教育部工程研究中心开放基金项目(20150408,AS201607);大学生创新创业训练计划项目(201510637085);重庆高校创新团队项目(CXTDX201601018)

Preliminary Research on Ultrasonic Assisted Extraction Technology of Polysaccharides in Ginkgo biloba Leaves

GUO Xiangqin1,2, CAI Na1, YANG Shanbin1,2, ZHOU Shiyang1,2, CHEN Yixin1, KE Daoyao1   

  1. 1. College of Chemistry, Chongqing Normal University, Chongqing 401331, China;
    2. Engineering Research Center of Biotechnology of Active Substances(Ministry of Education) in Chongqing Normal University, Chongqing 401331, China
  • Received:2016-10-16 Online:2017-11-30 Published:2017-12-08

摘要: 以银杏落叶为原料,采用超声波辅助水提醇沉的方法提取银杏落叶中的多糖,通过单因素试验和响应曲面法对银杏落叶中多糖提取的工艺进行初探。结果表明:最佳提取工艺条件为5.0 g银杏落叶粗粉,超声波功率600 W,液料比40∶1(mL∶g),提取温度84 ℃,提取时间50 min,多糖提取得率6.61 %。由于本研究只是对银杏落叶中多糖提取工艺的初步研究,因此选择提取的液料比时未深入考虑"少量多次,总液比尽量少"的提取原则。

关键词: 银杏落叶, 多糖, 超声波提取, 响应曲面法

Abstract: Polysaccharide was extracted from Ginkgo biloba fallen leaves by ultrasound-assisted water extraction and alcohol precipitation. The extraction technology was preliminarily studied by single factor and response surface method.The results showed that the optimum extraction conditions were as follows:G. biloba fallen leaves 5.0 g, ultrasound power 600 W, liquid/solid ratio 40:1(mL:g), extraction temperature 84 ℃, extraction time 50 min, the final polysaccharide yield was 6.61 %. Because this study was only a preliminary study on the extraction process of polysaccharides from the leaves of Ginkgo biloba, the extraction principle of "small amount of times and total liquid ratio is less" was not considered.

Key words: Ginkgo biloba fallen leaves, polysaccharide, ultrasonic extraction, response surface method

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