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生物质化学工程 ›› 2017, Vol. 51 ›› Issue (4): 1-7.doi: 10.3969/j.issn.1673-5854.2017.04.001

• 研究报告 •    下一篇

水溶性和碱溶性蔗渣木质素的分离与表征

彭锋, 付亘悫, 汪小君, 胡亚洁, 岳盼盼   

  1. 北京林业大学 林木生物质化学北京市重点实验室, 北京 100083
  • 收稿日期:2016-01-08 出版日期:2017-07-30 发布日期:2017-08-03
  • 作者简介:彭锋(1979-),男,四川仁寿人,教授,博士,主要从事植物资源开发利用的研究;E-mail:fengpeng@bjfu.edu.cn。
  • 基金资助:
    林业公益性行业科研专项(201404617);国家自然科学基金青年基金(31470417)

Isolation and Characterization of Water-soluble and Alkali-soluble Lignin from Bagasse

PENG Feng, FU Genque, WANG Xiaojun, HU Yajie, YUE Panpan   

  1. Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China
  • Received:2016-01-08 Online:2017-07-30 Published:2017-08-03

摘要: 以蔗渣为原料,经脱蜡后依次用H2O、1% NaOH和3% NaOH进行抽提,分别得到水溶性木质素L1和碱溶性木质素L2、L3,并对其理化性质和结构特征进行分析。通过硝基苯氧化法、高效液相色谱检测来确定蔗渣木质素的组分,通过凝胶渗透色谱检测蔗渣木质素的分子质量,利用紫外分光光度计和二维核磁共振推导出蔗渣木质素的结构单元和连接方式。结果表明,L1、L2和L3的得率(以绝干原料计)分别为0.4%、5.7%和3.7%,占蔗渣木质素总量的54.1%,而且水溶性木质素分子质量大于碱溶性木质素。L2和L3主要来自于蔗渣次生壁,富含紫丁香基单元和大量的非缩合醚结构。蔗渣木质素的结构单元间的主要连接方式是β-O-4'醚键,还有β-β'、β-5'和β-1'等碳碳结构。蔗渣木质素是由紫丁香基、愈创木基和少量对羟苯基结构单元组成的GSH型木质素,此外,还含有对香豆酸和阿魏酸,通过酯键与木质素相连。

关键词: 水溶性蔗渣木质素, 碱溶性蔗渣木质素, 结构特征, 核磁表征

Abstract: The lignin fractions(water-soluble lignin L1 and alkali-soluble lignin L2, L3)were extracted by H2O, 1% NaOH and 3% NaOH aqueous solution from the dewaxed bagasse. The components of three lignin fractions were analyzed by nitrobenzene oxidation and high performance liquid chromatography, the molecular weights were detected by gel permeation chromatography, and the structure units and linkages were studied by UV and 2D HSQC NMR. The results reflected that the yields of L1, L2 and L3 were 0.4%, 5.7% and 3.7%, and they occupied 54.1% of the original lignin.And the molecular weight of water-soluble lignin was higher than that of alkali-soluble lignin. Furthermore, the L2 and L3 were from the secondary wall of bagasse and the dominating constituents of them were syringyl units and large amounts of noncondensed ether structures. The lignin structural units were linked to each other mainly by β-O-4' ether bonds, β-β', β-5', β-1'. And the lignin was GSH lignin composed by syringyl, guaiacyl and little p-hydroxylphenyl units. The linkage between p-coumaric and lignin was ester bond, whereas the linkages between ferulic acid and lignin were both ether and ester bonds.

Key words: water-soluble bagasse lignin, alkali-soluble bagasse lignin, structural features, 2D-HSQC NMR

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