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生物质化学工程 ›› 2016, Vol. 50 ›› Issue (6): 9-16.doi: 10.3969/j.issn.1673-5854.2016.06.002

• 研究报告 • 上一篇    下一篇

氢氧化钠预处理对甘蔗渣酶解和发酵性能的影响

张宪宝1, 张腾1, 谢文化2, 朱明军1   

  1. 1. 华南理工大学 生物科学与工程学院, 广东 广州 510006;
    2. 广州甘蔗糖业研究所, 广东 广州 510316
  • 收稿日期:2016-03-08 出版日期:2016-11-30 发布日期:2016-12-03
  • 通讯作者: 朱明军(1969-),男,教授,博士生导师,研究方向为纤维素酒精及工业废弃物综合利用;E-mail:mjzhu@scut.edu.cn E-mail:mjzhu@scut.edu.cn
  • 作者简介:张宪宝(1988-),男,山东泰安人,硕士,主要从事生物质能源研究工作
  • 基金资助:
    国家自然科学基金资助项目(51278200,51478190);广东省自然科学基金重点项目(2014A030311014)

Impacts of NaOH Pretreatment on Enzymolysis and Fermentation Performance of Sugarcane Bagasse

ZHANG Xian-bao1, ZHANG Teng1, XIE Wen-hua2, ZHU Ming-jun1   

  1. 1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China;
    2. Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, China
  • Received:2016-03-08 Online:2016-11-30 Published:2016-12-03

摘要: 采用氢氧化钠预处理甘蔗渣,通过单因素和正交试验考察了不同预处理条件对甘蔗渣酶解和发酵性能的影响,并进一步分析了比表面积和木质素含量对酶解性能的影响。结果表明:预处理温度、氢氧化钠质量分数及预处理时间对酶解和发酵效率影响较为显著,最佳的预处理条件为:温度85℃、时间11 h、NaOH质量分数4.5%,在此优化条件下预处理的甘蔗渣,含纤维素56.46%,与原料相比提高了46.16%;半纤维素20.30%、Klason木质素5.79%,与原料相比分别降低了15.77%和72.87%,酶解36 h的还原糖得率为0.69 g/g(以甘蔗渣质量计)。经过氢氧化钠预处理后的甘蔗渣比表面积显著增加(由原料的0.07 m2/g最大可增加到1.07 m2/g),木质素显著降低,有利于提高酶解和发酵效率。当比表面积超过0.30 m2/g时,酶解初始速率和酶解效率达到平衡;当木质素低于11%时,酶解效率达到平衡。

关键词: 氢氧化钠预处理, 甘蔗渣, 酶解发酵

Abstract: The impacts of different pretreatment conditions on enzymolysis and fermentation performance of sugarcane bagasse(SCB) pretreated by NaOH were investigated by single factor analysis and orthogonal experimental design.The effects of specific surface area and lignin content on enzymolysis of SCB were further studied.The results showed that the pretreatment temperature,time and mass fraction of NaOH significantly affected the enzymolysis and fermentation efficiency of the pretreated SCB.The optimal conditions for NaOH pretreatment were mass fraction of NaOH 4.5%,11 h and 85℃.Under these conditions,the cellulose content of the pretreated SCB reached 56.46% and increased by 46.16% compared with the raw SCB. And the xylan and Klason lignin contents reached 20.30% and 5.79%,which decreased by 15.77% and 72.87%,respectively.The reducing sugar yield reached 0.69 g/g substrate after 36 h enzymolysis.The specific surface area of pretreated SCB significantly increased (the maximum value of 1.07 m2/g from 0.07 m2/g of the raw was obtained),while the lignin content decreased significantly.Both of them were beneficial for the enhancement of the enzymolysis and fermentation efficiency.It was found that when the specific surface area surpassed 0.30 m2/g,the initial enzymolysis rate and final efficiency could reach a balance.Besides,when the the lignin content was lower than 11%,the enzymolysis efficiency reached a balance.

Key words: NaOH pretreatment, sugarcane bagasse, enzymolysis and fermentation

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