欢迎访问《生物质化学工程》,

生物质化学工程 ›› 2016, Vol. 50 ›› Issue (4): 42-46.doi: 10.3969/j.issn.1673-5854.2016.04.008

• 研究报告 • 上一篇    下一篇

大孔树脂纯化栾树叶多酚工艺研究

李衍方1,2, 高彩霞3, 韩相恩1   

  1. 1. 中国矿业大学化工学院, 江苏 徐州 221116;
    2. 徐州市产品质量监督检验中心, 江苏 徐州 221000;
    3. 北京海德润制药有限公司, 北京 101113
  • 收稿日期:2015-12-29 出版日期:2016-07-31 发布日期:2016-10-08
  • 通讯作者: 韩相恩,教授,博士,博士生导师,研究领域:天然资源化学利用;E-mail:xiangenh@163.com。 E-mail:xiangenh@163.com
  • 作者简介:李衍方(1982-),女,江苏徐州人,工程师,博士生,主要从事天然资源化学利用工作
  • 基金资助:
    中央高校基本科研业务费专项基金(2015XKZD08)

Purification of Polyphenols from Koelreuteria paniculata Laxm. Leaves by Macroporous Resin

LI Yan-fang1,2, GAO Cai-xia3, HAN Xiang-en1   

  1. 1. China University of Mining and Technology, School of Chemical Engineering and Technology, Xuzhou 221116, China;
    2. Xuzhou Products Quality Supervision and Inspection Center, Xuzhou 221000, China;
    3. Beijing Haiderun Pharmaceutical Co. Ltd, Beijing 101113, China
  • Received:2015-12-29 Online:2016-07-31 Published:2016-10-08

摘要: 以栾树叶多酚提取物为原料,比较了7种大孔树脂对栾树叶多酚的静态吸附与解吸效果,结果表明AB-8树脂性能最佳,其24h静态吸附量为13.74mg/g,解吸率为98.35%,3h内达到吸附平衡与解吸平衡。AB-8树脂动态吸附较佳条件为上样液质量浓度为4g/L,上样液pH值为6,在此条件下吸附率为88.21%,动态洗脱较佳条件为洗脱剂乙醇体积分数为60%,洗脱速度为1mL/min,解吸率达到89.91%,在该条件下栾树叶总多酚经AB-8树脂纯化后,质量分数由50.36%增加到72.37%,回收率为86.83%。

关键词: 栾树叶多酚, 纯化, 大孔树脂

Abstract: The absorption and desorption behaviors of seven macroporous resins for polyphenols from Koelreuteria paniculata Laxm. were investigated. The 24h static adsorption capacity and desorption rate of AB-8 resin was 13.74mg/g and 98.35%, respectively. The static adsorption and desorption equilibrium time of AB-8 resin was both less than 3h. The optimum conditions of dynamic absorption and desorption by AB-8 were sample mass concentration 4g/L, sample pH value 6, and volume fraction of ethanol 60%, elution speed 1mL/min, respectively.Under these conditions, the adsorption rate was 88.21% and desorption rate was 89.91%.The purity of polyphenols from Koelreuteria paniculata Laxm. increased from 50.36% to 72.37% after purification using AB-8 resin and the recovery rate was 86.83%.

Key words: polyphenols of Koelreuteria paniculata Laxm. Leaves, purification, macroporous resin

中图分类号: