NaOH-Fenton试剂预处理桑木的纤维素酶分段酶水解研究

doi:10.3969/j.issn.1673-5854.2016.04.006

生物质化学工程 ›› 2016, Vol. 50 ›› Issue (4): 31-36.doi: 10.3969/j.issn.1673-5854.2016.04.006

NaOH-Fenton试剂预处理桑木的纤维素酶分段酶水解研究

王燕云^1,2, 吴凯^1,2, 雷福厚³, 杨静^1,2

1. 西南林业大学云南省木材胶黏剂及胶合制品重点实验室, 云南昆明 650224;
2. 西南林业大学材料工程学院, 云南昆明 650224;
3. 广西林产化学与工程重点实验室, 广西南宁 530006

收稿日期:2016-01-06 出版日期:2016-07-31 发布日期:2016-10-08
通讯作者: 杨静(1976-),女,博士,副教授,主要从事木质纤维素的生物降解与转化的研究;E-mail:kmjingyang@163.com。 E-mail:kmjingyang@163.com
作者简介:王燕云(1991-),女,江苏苏州人,硕士生,研究方向为生物质能源;E-mail:1394938725@qq.com
基金资助:
云南省应用基础研究计划项目（2011FZ137）；国家自然科学基金资助项目（31260162）；广西林产化学与工程重点实验室开放课题（GXFC13-06）

Process Resrarch of Multi-stage Enzymatic Hydrolysis of NaOH-Fenton Pretreated Mulberry Wood

WANG Yan-yun^1,2, WU Kai^1,2, LEI Fu-hou³, YANG Jing^1,2

1. Yunnan Provincial Key Laboratory of Wood Adhesives and Glued Products, Southwest Forestry University, Kunming 650224, China;
2. College of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China;
3. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China

Received:2016-01-06 Online:2016-07-31 Published:2016-10-08

摘要/Abstract

摘要： 采用分段酶水解木质纤维原料的方法，以NaOH-Fenton试剂预处理桑木为原料，通过在反应过程中及时移除葡萄糖和纤维二糖，减轻产物的抑制作用，最终达到提高酶水解得率和缩短酶解反应时间的目的。实验结果表明：纤维素酶用量为15FPIU/g（以纤维素计，下同）时，在三段（8+8+8h）水解过程中，经第一段水解，纤维素酶反应速率从1.25g/（L·h）提高到2.21g/（L·h），第二段水解后，酶反应速率为1.54g/（L·h），比未分段水解的酶反应速率提高了73%；当纤维素酶用量为40FPIU/g时，三段（8+8+8h）水解得率增至88.08%；三段（8+8+8h）水解充分利用了酶解残渣上的结合酶进行后续水解。对纤维素酶在预处理桑木上的吸附情况进行研究，发现桑木经NaOH-Fenton试剂预处理后，对纤维素酶的最大吸附量为8.08mg/g，预处理增加了纤维素酶与桑木间的吸附位点。

关键词: 纤维素酶, 酶水解, 酶反应速率, 纤维素吸附

Abstract: During the multi-stage enzymatic hydrolysis of lignocellulosic materials, the product inhibitions were reduced by the removal of end products at each stage to enhance the hydrolysis efficiency and shorten the hydrolysis time with NaOH-Fenton pretreated mulberry as raw material. During the three-stage (8+8+8h) hydrolysis, as the enzymatic dosage was 15FPIU/g(calculated by the mass of cellulose, similarly here in after), enzymatic average reaction rate increased from 1.25 to 2.21g/(L·h) at the first stage, and after the second stage, it reached 1.54g/(L·h) and increased rapidly by 73% comparing with that of unsegmented hydrolysis.When enzymatic dosage was 40FPIU/g, the three-stage (8+8+8h) hydrolysis could reach 88.08%.Multi-stage hydrolysis made full use of the absorbed cellulase on substrate.The classical Langmuir adsorption isotherm was used to fit the cellulase enzyme adsorption on substrates.The adsorption capacity of cellulase on NaOH-Fenton pretreated mulberry was studied with the maximum adsorption capacity of 8.08mg/g substrate.The adsorbed sites were enhanced in the pretreatment process.

Key words: cellulase, enzymatic hydrolysis, enzymatic reaction rate, cellulose adsorption

中图分类号:

TQ35

王燕云, 吴凯, 雷福厚, 杨静. NaOH-Fenton试剂预处理桑木的纤维素酶分段酶水解研究[J]. 生物质化学工程, 2016, 50(4): 31-36.

WANG Yan-yun, WU Kai, LEI Fu-hou, YANG Jing. Process Resrarch of Multi-stage Enzymatic Hydrolysis of NaOH-Fenton Pretreated Mulberry Wood[J]. bce, 2016, 50(4): 31-36.

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NaOH-Fenton试剂预处理桑木的纤维素酶分段酶水解研究

Process Resrarch of Multi-stage Enzymatic Hydrolysis of NaOH-Fenton Pretreated Mulberry Wood

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