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生物质化学工程 ›› 2015, Vol. 49 ›› Issue (6): 27-30.doi: 10.3969/j.issn.1673-5854.2015.06.006

• 研究报告 • 上一篇    下一篇

山核桃外果皮的漆酶改性处理研究

王进, 韩素芳, 张飞英, 刘亚群   

  1. 浙江省林业科学研究院, 浙江 杭州 310023
  • 收稿日期:2015-06-24 出版日期:2015-11-30 发布日期:2016-03-17
  • 作者简介:王进(1984-),男,湖北通山人,助理研究员,博士生,研究方向:木材科学与技术;E-mail:whuwj@sina.com。
  • 基金资助:
    浙江省公益技术研究农业项目(2013C32104)

Laccase Treatment of Cathay Hickory Ectocarp

WANG Jin, HAN Su-fang, ZHANG Fei-ying, LIU Ya-qun   

  1. Zhejiang Forestry Academy, Hangzhou 310023, China
  • Received:2015-06-24 Online:2015-11-30 Published:2016-03-17

摘要: 为高效利用山核桃外果皮资源,对山核桃外果皮进行了漆酶改性处理。测定了改性处理前后样品的化学成分含量,采用SEM观测改性前后样品的微观结构,FT-IR和13C NMR分析改性处理前后样品的化学结构变化。结果表明:漆酶处理后,山核桃外果皮木质素和抽提物含量降低,粉末的分散效果更好,表面更多的木质素裸露出来。山核桃外果皮以愈创木基和紫丁香基结构单元为主的木质素结构单元,在漆酶处理过程中发生β-O-4断裂产生较多的活性氧类自由基,使山核桃外果皮表面获得较多的活性基团,从而具有更高的反应活性。

关键词: 山核桃外果皮, 漆酶活化, SEM, FT-IR, 13C NMR

Abstract: In order to use the resources of the cathay hickory exocarp efficiently, the ectocarp powder was treated by laccase. The chemical compositions of the samples were determined, the microstructures of the lacasse treated and untreated ectocarp were observed by SEM, and the chemical structures of the samples were analyzed by using FT-IR and 13C NMR. The results showed that after laccase activation, the contents of lignin and extracts of cathay hickory ectocarps de creased and the powders were dispersed better, moreover, there was more exposed lignin on the surface. And the guaiacyl and syringyl lignin were degraded by β-O-4 fracture, and meanwhile more reactive oxygen species free radicals produced. This made the ectocarp surface to obtain more active groups and had higher reaction activity.

Key words: cathay hickory ectocarp, laccase activation, FT-IR, 13C NMR, SEM

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