响应曲面法优化小麦秸秆纤维素酶水解条件

doi:10.3969/j.issn.1673-5854.2015.02.008

生物质化学工程 ›› 2015, Vol. 49 ›› Issue (2): 39-46,52.doi: 10.3969/j.issn.1673-5854.2015.02.008

响应曲面法优化小麦秸秆纤维素酶水解条件

张伟¹, 张琦², 阮馨怡², 王欣泽², 孔海南², 林燕²

1. 上海环科环境认证有限公司, 上海 200233;
2. 上海交通大学环境科学与工程学院, 上海 200240

收稿日期:2014-04-14 修回日期:2014-12-29 出版日期:2015-03-30 发布日期:2015-04-07
通讯作者: 林燕(1976-),女,上海人,副教授,博士,硕士生导师,研究领域为生物质能源化利用及流域水体富营养化生态治理; E-mail:linyansjtu@126.com。 E-mail:linyansjtu@126.com
作者简介:张伟(1987-),男,江苏苏州人,硕士,主要从事清洁生产审核及生物质能源化利用研究;E-mail:nemosjtu@sina.com
基金资助:
国家水体污染控制与治理科技重大专项(2012ZX07105-003)

Optimization of Enzymatic Hydrolysis of Wheat Straw by Using Response Surface Methodology

ZHANG Wei¹, ZHANG Qi², RUAN Xin-yi², WANG Xin-ze², KONG Hai-nan², LIN Yan²

1. Shanghai Huanke Environmental Certification Ltd., Co., Shanghai 200233, China;
2. School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

Received:2014-04-14 Revised:2014-12-29 Online:2015-03-30 Published:2015-04-07

摘要/Abstract

摘要： 利用响应曲面试验设计方法(RSM),选择底物质量分数、酶投加量、温度、pH值及水解时间为试验因子,还原糖(RS)产率为响应值,考察小麦秸秆纤维素酶水解过程中各影响因子对还原糖产率的影响,对小麦秸秆纤维素酶水解条件进行优化。结果表明,所考察的5个影响因子对还原糖产率均具有显著影响(p<0.05)。所得回归方程R² 值为 0.946 9,p<0.05,变异系数(C_V)值为4.37%,足够精度值为26.396,说明模型高度显著,可以在设计范围内对响应值进行预测。模型预测最佳水解条件为底物质量分数8.0%,酶投加量为35 FPU/g(以秸杆质量计),温度50 ℃,pH值5.4,水解时间96 h。利用最优水解条件进行验证试验,所得还原糖产率为60.73%,水解液中葡萄糖和木糖质量浓度分别为31.84和 16.74 g/L。

关键词: 小麦秸秆, 纤维素酶水解, 响应曲面法, 还原糖

Abstract: With an aim to optimize the conditions of enzymatic hydrolysis of wheat straw,the parameters including solid content,enzyme loading,temperature,pH,and hydrolysis time were investigated by means of response surface methodology(RSM) with the reducing sugar(RS) yield as response value.The results showed that all the parameters had significant effects(p<0.05) on the reducing sugar yield.A well fitted regression equation with R² value of 0.946 9,p<0.05,coefficient variability(C_v) value of 4.37%,and adequate precision value of 26.396 indicated that the developed model was significant and could be used to navigate the designed space and predict the response.The predicted optimum conditions for enzymatic hydrolysis were solid content of 8.0%,enzyme loading of 35 FPU/g,temperature of 50 ℃,pH of 5.4,and hydrolysis time of 96 h.The experimental results showed that under the optimum conditions the corresponding RS yield was 60.73% with glucose and xylose concentrations of 31.84 and 16.74 g/L,respectively.Further,the results obtained in this research showed a high RS yield with high initial solid content,which would significantly improve the ethanol concentration,reduce the subsequently distillation costs,and improve the commercial potential of the lignocellulosic bioethanol production.

Key words: wheat straw, enzymatic hydrolysis, response surface methodology, reducing sugar

中图分类号:

张伟, 张琦, 阮馨怡, 王欣泽, 孔海南, 林燕. 响应曲面法优化小麦秸秆纤维素酶水解条件[J]. 生物质化学工程, 2015, 49(2): 39-46,52.

ZHANG Wei, ZHANG Qi, RUAN Xin-yi, WANG Xin-ze, KONG Hai-nan, LIN Yan. Optimization of Enzymatic Hydrolysis of Wheat Straw by Using Response Surface Methodology[J]. bce, 2015, 49(2): 39-46,52.

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响应曲面法优化小麦秸秆纤维素酶水解条件

Optimization of Enzymatic Hydrolysis of Wheat Straw by Using Response Surface Methodology

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