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生物质化学工程 ›› 2019, Vol. 53 ›› Issue (6): 33-38.doi: 10.3969/j.issn.1673-5854.2019.06.006

• 研究报告 • 上一篇    下一篇

离子色谱法测毛竹竹叶单糖和糖醛酸组成

张强(),季红福,周燕,范煜,葛青,肖竹钱,毛建卫*()   

  1. 浙江省农产品化学与生物加工技术重点实验室, 浙江省农业生物资源生化制造协同创新中心, 浙江科技学院 生物与化学工程学院, 浙江 杭州 310023
  • 收稿日期:2018-12-20 出版日期:2019-11-30 发布日期:2019-12-10
  • 通讯作者: 毛建卫 E-mail:zq15268847626@163.com;zjhzmjw@163.com
  • 作者简介:张强(1994—),男,浙江台州人,硕士生,主要从事生物活性物质的分离纯化及分析工作; E-mail: zq15268847626@163.com
  • 基金资助:
    浙江省重点研发项目(2015C02031);浙江省农业生物资源生化制造协同创新中心开放基金(2016KF0035);浙江省农业生物资源生化制造协同创新中心开放基金(2016KF0110)

Monosaccharide and Alduronic Acid Composition of Bamboo Leaves by Ion Chromatography

Qiang ZHANG(),Hongfu JI,Yan ZHOU,Yu FAN,Qing GE,Zhuqian XIAO,Jianwei MAO*()   

  1. Zhejiang Provincial Key Lab. for Chem & Bio Processing Technology of Farm Product, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China
  • Received:2018-12-20 Online:2019-11-30 Published:2019-12-10
  • Contact: Jianwei MAO E-mail:zq15268847626@163.com;zjhzmjw@163.com
  • Supported by:
    浙江省重点研发项目(2015C02031);浙江省农业生物资源生化制造协同创新中心开放基金(2016KF0035);浙江省农业生物资源生化制造协同创新中心开放基金(2016KF0110)

摘要:

以离子色谱建立了一种简便、快速、准确测定木质纤维基单糖组成的方法。采用Dionex ICS-5000+离子色谱仪,以CarboPACTM PA10 (4 mm×250 mm)阴离子交换柱为分离柱,脉冲安培检测器进行检测,氢氧化钠-乙酸钠进行梯度洗脱,同时检测7种常见单糖和2种糖醛酸,结果表明:在各自线性范围内线性关系良好(R2≥0.999 0),精密度相对标准偏差(RSD)均小于3%,加标回收率98.53%~118.63%,重复性RSD均小于1.16%,检测限为0.01~ 1 mg/L,定量限为0.05~3 mg/L。对竹叶水解液的单糖组成进行了测定,结果表明:竹叶水解液是由阿拉伯糖、半乳糖、葡萄糖和木糖组成,相应单糖的物质的量比为n(阿拉伯糖) : n(半乳糖) : n(葡萄糖) : n(木糖)=2.9 : 0.7 : 8.2 : 9.7。说明该方法可用于木质纤维基单糖的检测。

关键词: 离子色谱-脉冲安培法, 木质纤维素, 单糖, 糖醛酸, 方法学研究

Abstract:

A simple, rapid and accurate method for the determination of monosaccharide compositions from lignocellulose byionchromatographywas established. The HPAEC-PAD method was established to simultaneously detect 7 different monosaccharides and two types of alduronic acid. Dionex ICS-5000+ with CarboPACTMPA10(4 mm×250 mm) column and pulsed amperometric detection was involved in this method. This method could be simultaneously qualitatively and quantitatively analysed by the gradient elution of NaOH and CH3COONa.The results showed that the method has good linearity (R2≥0.999 0), high precision (RSD < 3%), adequate (98.53%-118.63% recovery) and good reproducibility (RSD < 1.16%)within the range. The limitions of quantitation and detection were 0.05-3.0 mg/L and 0.01-1 mg/L, respectively. The method was applied to determine the monosaccharide compositions from bamboo leaves hydrolysate.The results showed that bamboo leaves hydrolysate was composed of arabinose, galactose, glucose and xylose.The mole ratio of monosaccharide to n(arabinose) : n(galactose) : n(glucose) : n(xylose)=2.9 : 0.7 : 8.2 : 9.7.This method is successful and can be used for the determination of monosaccharides in lignocellulose.

Key words: HPAEC-PAD method, lignocelluloses, monosaccharide, uronic acid, methodology study

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