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生物质化学工程 ›› 2020, Vol. 54 ›› Issue (1): 55-59.doi: 10.3969/j.issn.1673-5854.2020.01.009

• 研究报告 • 上一篇    下一篇

超声波辅助纤维素酶水解制备纳米纤维素

卢琳娜,卢麒麟*()   

  1. 闽江学院 服装与艺术工程学院; 福建省新型功能性纺织纤维及材料重点实验室, 福建 福州 350108
  • 收稿日期:2019-01-28 出版日期:2020-01-30 发布日期:2020-02-11
  • 通讯作者: 卢麒麟 E-mail:qilinlu@mju.edu.cn
  • 作者简介:卢琳娜(1993-),女,河南周口人,助理实验师,硕士,主要从事功能性生物质材料研究
  • 基金资助:
    福建省教育厅科研课题(JT180389);福建省新型功能性纺织纤维及材料重点实验室开放基金项目(FKLTFM1703);闽江学院引进人才项目(MJY18010)

Preparation of Nanocellulose by Cellulase Hydrolysis Under Sonication-assisted

Linna LU,Qilin LU*()   

  1. Clothing and Design Faculty, Minjiang University; Fujian Key Laboratory of Novel Functional Textile Fibers and Materials, Fuzhou 350108, China
  • Received:2019-01-28 Online:2020-01-30 Published:2020-02-11
  • Contact: Qilin LU E-mail:qilinlu@mju.edu.cn
  • Supported by:
    福建省教育厅科研课题(JT180389);福建省新型功能性纺织纤维及材料重点实验室开放基金项目(FKLTFM1703);闽江学院引进人才项目(MJY18010)

摘要:

以人纤浆为原料,利用超声波辅助纤维素酶水解制备纳米纤维素(NCC),在单因素试验基础上,采用正交试验优化NCC的制备条件,并通过透射电镜(TEM)、X射线衍射(XRD)、红外光谱(FT-IR)、热重(TG)和Zeta电位测定对NCC的结构和性能进行了分析与表征。研究结果表明:NCC的最佳制备条件为酶用量为人纤浆质量的7%、50℃条件下酶解反应10 h,此时纳米纤维素的得率可达62.3%。TEM表征显示制备的纳米纤维素呈短棒状,纳米纤维素之间相互交织形成网络结构;XRD分析表明纳米纤维素的晶体结构并未发生改变,仍为纤维素Ⅰ型,结晶度由人纤浆的54.2%增大到73%;FT-IR分析显示纳米纤维素仍保持天然纤维素的化学结构;热重分析表明纳米纤维素的热稳定性较纤维素原料显著提高;Zeta电位测试结果表明纳米纤维素在水介质中具有良好的分散稳定性。

关键词: 纳米纤维素, 纤维素酶, 超声波, 绿色, 高效

Abstract:

Nanocellulose(NCC) was prepared with cellulose pulp as raw material by using cellulase hydrolysis under sonication-assisted technology. Orthogonal test was used to optimize the preparation of NCC on the basis of single factor test. The structure and properties of NCC were analyzed and characterized by transmission electron microscope(TEM), X-ray diffraction(XRD), infrared spectroscopy(FT-IR), thermogravimetry(TG) and Zeta potential measurement. The results showed that the yield of 62.3% for NCC could be achieved when the cellulase dosage was 7%, reaction temperature was 50℃ and reaction time was 10 h. TEM image showed that the prepared NCC was short rod-like, which interacted with each other to form a web-like network. XRD results suggested that the crystal structure of NCC was cellulose I, and the crystallinity increased from 54.2% to 73%. FT-IR implied that the original chemical structure of NCC was not altered. TGA analysis indicated that the thermal stability of NCC was significantly improved compared with that of cellulose pulp. The Zeta potential test showed that NCC suspension had good dispersion stability in aqueous medium.

Key words: nanocellulose, cellulase, sonication, green, high-efficient

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