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生物质化学工程 ›› 2016, Vol. 50 ›› Issue (6): 49-55.doi: 10.3969/j.issn.1673-5854.2016.06.008

• 研究报告 • 上一篇    下一篇

间歇出酶/出菌丝的培养方式对里氏木霉产纤维素酶的影响

蒋露, 王步成, 白合超, 勇强, 余世袁   

  1. 南京林业大学 林木遗传与生物技术省部共建教育部重点实验室, 江苏 南京 210037
  • 收稿日期:2016-01-06 出版日期:2016-11-30 发布日期:2016-12-03
  • 通讯作者: 余世袁,教授,博士生导师,主要从事生物质能源转化研究;E-mail:syu@njfu.edu.cn E-mail:syu@njfu.edu.cn
  • 作者简介:蒋露(1990-),女,江苏宜兴人,硕士生,研究方向为生物质能源科学与技术;E-mail:544915512@qq.com
  • 基金资助:
    “十二五”国家科技支撑计划资助(2015BAD15B09)

Influence of Periodic Enzyme and Mycelia Recovery on Cellulase Production by Trichoderma reesei

JIANG Lu, WANG Bu-cheng, BAI He-chao, YONG Qiang, YU Shi-yuan   

  1. Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
  • Received:2016-01-06 Online:2016-11-30 Published:2016-12-03

摘要: 以微晶纤维素和淀粉水解液作为碳源生产纤维素酶,在里氏木霉Rut C30分批补料生产纤维素酶的过程中通过间歇取出部分酶的培养方式保护纤维素酶。实验结果显示:采用间歇出酶培养方式,在分批补料3 d后,每1、2或者3天取出部分酶液。当平均每天取出15%的酶液,酶活显著增加,总纤维素酶酶活较单纯分批补料提高26.5%~32.6%,而总β-葡萄糖苷酶酶活提高超过46%;采用间歇出酶出菌丝培养方式,当每天取出15%的酶液时,纤维素酶产量比分批补料增长35.4%,去除和酶液等量的菌丝,酶活和分批补料相比增长32.5%,而且这两种培养方式所生产的纤维素酶的酶解效率高达82%,远超商品酶Celluclast(62.03%)。

关键词: 纤维素酶, 分批补料, &beta, -葡萄糖苷酶, 里氏木霉

Abstract: The cellulase was produced by fed-batch cultivation of Trichoderma reesei Rut C30 with microcrystalline cellulose and starch hydrolysate as carbon source under the protection of periodic enzyme recovery.The results showed that after three days of fed-batch cultivation,cellulase was partially recovered 15%,30% or 45% at every 1,2 or 3 days.The total enzyme activites of cellulase and β-glucosidase obtained by the methods significantly increased by 26.5%-32.6% and over 46% more than that of fed-bath.Mycelia could also be removed to avoid infection.When enzymes were recovered 15% every day, the cellulase production increased by 35.4% (without mycelia removal) and 32.5% (with 15% mycelial removal) compared with those of normal fed-batch cultivation.A high hydrolytic ability of the produced cellulase with enzyme and mycelia recovery was also detected and the hydrolysis efficiency could reach more than 82%,far more than that of Celluclast(62.03%).

Key words: cellulase, fed-batch culture, β-glucosidase, Trichoderma reesei

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