典型预处理对甘草渣酶解产糖及其结构的影响

doi:10.3969/j.issn.1673-5854.2023.03.005

生物质化学工程 ›› 2023, Vol. 57 ›› Issue (3): 31-38.doi: 10.3969/j.issn.1673-5854.2023.03.005

典型预处理对甘草渣酶解产糖及其结构的影响

刘梦佳¹^,², 魏高杰¹^,³, 于晨曦¹^,⁴, 邢建民¹, 刘新育², 杨茂华¹^,*()

1. 中国科学院过程工程研究所, 中国科学院绿色过程与工程重点实验室, 北京 100190
2. 河南农业大学生命科学学院, 河南郑州 450002
3. 河北科技大学环境科学与工程学院, 河北石家庄 050091
4. 河北科技大学食品与生物学院, 河北石家庄 050091

收稿日期:2022-04-29 出版日期:2023-05-30 发布日期:2023-05-31
通讯作者: 杨茂华 E-mail:mhyang@ipe.ac.cn
作者简介:杨茂华, 硕士生导师, 研究领域: 绿色生物催化与合成生物技术研究; E-mail: mhyang@ipe.ac.cn
刘梦佳(1995—), 女, 河南郑州人, 硕士生, 从事有机固废资源化利用研究

Effects of Typical Pretreatments on Sugar Production by Enzymatic Hydrolysis and Structural Changes of Licorice Root Residue

Mengjia LIU¹^,², Gaojie WEI¹^,³, Chenxi YU¹^,⁴, Jianmin XING¹, Xinyu LIU², Maohua YANG¹^,*()

1. Institute of Process Engineering, Chinese Academy of Sciences; Key Laboratory of Green Process and Engineering, Chinese Academy of Sciences, Beijing 100190, China
2. College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
3. College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050091, China
4. College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050091, China

Received:2022-04-29 Online:2023-05-30 Published:2023-05-31
Contact: Maohua YANG E-mail:mhyang@ipe.ac.cn

摘要/Abstract

摘要：

采用稀酸、水热、离子液体和蒸汽爆破4种典型预处理方式处理甘草渣, 通过HPLC、SEM、FT-IR、XRD分析, 比较不同预处理方法对甘草渣产糖及结构的影响。研究结果表明: 离子液体预处理对甘草渣中纤维素作用明显, 酶水解液中葡萄糖、木糖和木二糖含量最高, 其纤维素得率达到77.08%±2.12%, 是未处理的2.05倍; 水热预处理对甘草渣中半纤维素作用明显, 预处理液中木糖、阿拉伯糖和木二糖含量最多, 半纤维素得率最高, 达到82.37%±1.13%, 是未处理的8.90倍; 蒸汽爆破预处理对甘草渣中木质素作用明显, 预处理液中葡萄糖得率最高。结构分析结果与组分分析结果相一致, 相比于其他预处理方式, 离子液体预处理过程有效打破了甘草渣纤维结构, 更有利于甘草渣的进一步高值化利用。

关键词: 预处理, 还原糖, 木寡糖, 酶解效率

Abstract:

Four typical pretreatment methods of dilute acid, hydrothermal treatment, ionic liquid and steam blasting were used to treat licorice residue. The effects of different pretreatment methods on sugar production and structure of licorice residue were compared by high performance liquid chromatography, scanning electron microscope, fourier transform infrared spectrometer and X-ray diffraction analysis. The results showed that ionic liquid pretreatment had obvious effect on cellulose in licorice residue, and the contents of glucose, xylose and xylodisaccharide in the enzymatic hydrolysate were the highest, and the enzymolysis rate reached 77.08%±2.12%, which was 2.05 times than that of untreated enzymatic hydrolysis rate. Hydrothermal pretreatment had obvious effects on hemicellulose licorice residue, and the contents of xylose, arabinose and xylodisaccharide in the pretreatment solution were the highest. The enzymatic hydrolysis efficiency of hemicellulum was the highest, reaching 82.37%±1.13%, which was 8.90 times than that of untreated enzymolysis. Steam blasting pretreatment had obvious effect on licorice residue lignin, and the glucose yield in the pretreatment solution was the highest. The structural analysis results were consistent with the component analysis results. Compared with other pretreatment, the lignocellulose structure of licorice residue was effectively broken in the ionic liquid pretreatment process, which was more conducive to the further high-value utilization of licorice residue.

Key words: pretreatments, reducing sugar, xylo-oligosaccharide, enzymatic hydrolysis efficiency

中图分类号:

TQ35

刘梦佳, 魏高杰, 于晨曦, 邢建民, 刘新育, 杨茂华. 典型预处理对甘草渣酶解产糖及其结构的影响[J]. 生物质化学工程, 2023, 57(3): 31-38.

Mengjia LIU, Gaojie WEI, Chenxi YU, Jianmin XING, Xinyu LIU, Maohua YANG. Effects of Typical Pretreatments on Sugar Production by Enzymatic Hydrolysis and Structural Changes of Licorice Root Residue[J]. Biomass Chemical Engineering, 2023, 57(3): 31-38.

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预处理方式 pretreatment method	纤维素 cellulose	半纤维素 hemicellulose	木质素 lignin	半纤维素降解率 hemicellulose removal rate	木质素降解率 lignin removal rate
未预处理 not pretreated	46.58±1.24	13.36±1.08	26.42±1.10
离子液体 ionic liquid	40.40±1.07	6.01±0.84	17.09±0.54	55.23±2.67	35.29±0.65
水热 hydrothermal	47.39±1.32	2.32±0.63	25.4±0.90	82.90±3.33	3.84±0.60
稀酸 dilute acid	46.61±0.96	2.9±0.78	22.94±0.28	78.63±4.11	13.07±2.56
蒸汽爆破 steam explosion	46.72±0.70	5.9±0.66	15.38±0.80	55.95±1.38	41.81±0.61

预处理方式 pretreatment method	葡萄糖 glucose	木糖 xylose	阿拉伯糖 arabinose	木二糖 xylobiose	木三糖-木七糖¹⁾ xylotriose- xyloheptaose
离子液体 ionic liquid	1.96±0.12	1.48±0.59	0.19±0.04	1.03±0.11	1.23±0.14
水热 hydrothermal	2.7±0.18	7.61±1.01	1.83±0.09	3.49±0.23	4.23±0.42
稀酸 dilute acid	4.65±0.27	3.73±0.83	0.72±0.02	3.41±0.22	4.84±0.39
蒸汽爆破 steam explosion	6.75±0.61	1.99±0.33	0.32±0.09	1.67±0.14	2.09±0.23

预处理方式 pretreatment method	葡萄糖 glucose	木糖 xylose	阿拉伯糖 arabinose	木二糖 xylobiose	木三糖-木七糖 cellulase hydrolysis rate	纤维素得率 cellulose yield	半纤维素得率 hemicellulose yield
未预处理 not pretreated	33.89±0.47	5.27±0.31	2.60±0.26	0.34±0.06	1.83±0.35	37.64±1.03	9.25±0.88
离子液体 ionic liquid	60.20±1.12	14.48±0.19	4.42±0.6	7.60±0.17	8.84±0.37	77.08±2.12	49.68±1.28
水热 hydrothermal	41.64±0.68	9.50±0.11	2.57±0.29	4.38±0.13	5.26±0.24	45.36±2.43	82.37±1.13
稀酸 dilute acid	50.66±0.99	7.79±0.08	1.86±0.24	3.41±0.13	5.29±0.35	56.22±1.84	52.72±1.31
蒸汽爆破 steam explosion	53.72±1.06	12.38±0.12	5.50±0.69	6.12±0.18	8.06±0.41	59.47±1.96	47.64±1.65

预处理方式 pretreatment method	I_cr
预处理方式 pretreatment method	水解前 before hydrolysis	水解后 after hydrolysis
未预处理 not pretreated	37.64±1.03	42.38±0.88
离子液体 ionic liquid	17.06±1.72	17.71±2.28
水热 hydrothermal	46.61±1.43	58.52±2.63
稀酸 dilute acid	55.13±1.44	49.08±1.91
蒸汽爆破 steam explosion	43.48±0.81	57.19±1.95

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典型预处理对甘草渣酶解产糖及其结构的影响

Effects of Typical Pretreatments on Sugar Production by Enzymatic Hydrolysis and Structural Changes of Licorice Root Residue

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