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生物质化学工程 ›› 2021, Vol. 55 ›› Issue (3): 42-46.doi: 10.3969/j.issn.1673-5854.2021.03.007

• 研究报告 • 上一篇    下一篇

皂荚多糖的普鲁兰酶脱分支改性研究

朱昌玲(), 雷鹏, 张峰伦, 张焕仕()   

  1. 中华全国供销合作总社南京野生植物综合利用研究所, 江苏 南京 211111
  • 收稿日期:2020-05-07 出版日期:2021-05-30 发布日期:2021-05-20
  • 通讯作者: 张焕仕 E-mail:2289230204@qq.com;zhanghuanshi@126.com
  • 作者简介:张焕仕, 副研究员, 博士, 研究领域为土壤生态学与植物资源利用; E-mail: zhanghuanshi@126.com
    朱昌玲(1968-), 女, 重庆人, 高级工程师, 研究方向为生物工程; E-mail: 2289230204@qq.com
  • 基金资助:
    国家重点研发计划资助项目(2016YFD0600803)

Debranching Modification of Pullulanase of Gleditsia sinensis Polysaccharide

Changling ZHU(), Peng LEI, Fenglun ZHANG, Huanshi ZHANG()   

  1. Nanjing Institute for Comprehensive Utilization of Wild Plant China CO-OP, Nanjing 211111, China
  • Received:2020-05-07 Online:2021-05-30 Published:2021-05-20
  • Contact: Huanshi ZHANG E-mail:2289230204@qq.com;zhanghuanshi@126.com

摘要:

以皂荚多糖(GSP)为原料,考察了普鲁兰酶对皂荚多糖的脱支效应,并研究了改性皂荚多糖(MGSP)胶与黄原胶的复配性能。结果表明:皂荚多糖脱支改性处理6 h后获得了含半乳糖23.4%的改性皂荚多糖(GSP含半乳糖28.2%),且普鲁兰酶修饰并未改变多糖主链的主体结构和糖苷键的构型,只是降低了皂荚多糖分子链中侧链半乳糖的含量;改性皂荚多糖与黄原胶复配时,呈现出了显著的协同增效效应,并呈现出了一种较高弹性强凝胶的特性,复配比例对复配胶的性能起着至关重要的作用,当改性皂荚多糖与黄原胶的质量比为6:4时,复配胶的弹性模量最大(112.3 Pa),同时,复配胶的黏性模量受复配比例的影响则相对更大一些。

关键词: 普鲁兰酶, 脱分支改性, 皂荚多糖

Abstract:

The debranching effect of pullulanase on Gleditsia sinensis polysaccharide(GSP) was investigated, and the compounding properties of modified Gleditsia sinensis polysaccharide(MGSP) gum and xanthan gum were studied. The results showed that MGSP with 23.4% galactose content was obtained after treating for 6 h(the content of galactose in GSP was 28.2%), and pullulanase modification did not change the main structure and glycosidic bond configuration of the main chain of the polysaccharide, but reduced the galactose content of the side chain in the molecular chain of GSP; when the MGSP was mixed with xanthan gum, it showed a significant synergistic effect, and showed the characteristic of strong gel with high elasticity. When the MGSP was combined with xanthan gum, the compound ratio played a key role in the performance of the compound, when the mass ratio of MGSP and xanthan gum was 6:4, the elastic modulus of the compound gum was the largest(112.3 Pa), and the effect of the compound ratio on the viscosity modulus of the compound gum was relatively large.

Key words: pullulanase, debranching, GSP

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