向日葵盘精油的化学成分及抗氧化活性

doi:10.3969/j.issn.1673-5854.2020.01.007

生物质化学工程 ›› 2020, Vol. 54 ›› Issue (1): 43-48.doi: 10.3969/j.issn.1673-5854.2020.01.007

向日葵盘精油的化学成分及抗氧化活性

何昶毅¹,高潇涵¹,李泽文¹,刘玲玲²,孙彤彤²,陈小强^2,^3,*()

1. 东北林业大学生命科学学院, 黑龙江哈尔滨 150040
2. 东北林业大学化学化工与资源利用学院, 黑龙江哈尔滨 150040
3. 西藏农牧学院资源与环境学院, 西藏林芝 860000

收稿日期:2019-08-20 出版日期:2020-01-30 发布日期:2020-02-11
通讯作者: 陈小强 E-mail:cxqnefu@126.com
作者简介:何昶毅(1999-),男,山西临汾人,本科生,主要从事生物学研究工作
基金资助:
东北林业大学大学生创新训练项目(201910225336);黑龙江省博士后科研启动金项目(LBH-Q15009);黑龙江省自然科学基金项目(LH2019C006)

Compositions of the Essential Oil of Helianthus annuus Head and Its Antioxidant Activities

Changyi HE¹,Xiaohan GAO¹,Zewen LI¹,Lingling LIU²,Tongtong SUN²,Xiaoqiang CHEN^2,^3,*()

1. College of Life Science, Northeast Forestry University, Harbin 150040, China
2. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
3. College of Resources and Environment, Tibet Agriculture & Animal Husbandry University, Nyingchi 860000, China

Received:2019-08-20 Online:2020-01-30 Published:2020-02-11
Contact: Xiaoqiang CHEN E-mail:cxqnefu@126.com
Supported by:
东北林业大学大学生创新训练项目(201910225336);黑龙江省博士后科研启动金项目(LBH-Q15009);黑龙江省自然科学基金项目(LH2019C006)

摘要/Abstract

摘要：

为明晰采收后向日葵盘精油的成分及其体外抗氧化活性，应用水蒸气蒸馏法提取采收后向日葵盘精油，利用气相色谱-质谱联用法（GC-MS）分析其化学成分，通过DPPH自由基和ABTS自由基清除法对精油的体外抗氧化活性进行测定。结果显示：从向日葵盘精油中共鉴定出49种化学成分（占精油总量91.16%），其中α-蒎烯（28.22%）、柠檬烯（6.55%）、α-蛇麻烯（4.99%）、反式-β-金合欢烯（3.58%）和莰烯（3.39%）为其主要成分。向日葵盘精油具有一定的体外抗氧化活性，其清除DPPH自由基、ABTS自由基的半数有效浓度（EC₅₀）值分别为0.47、0.30 g/L，明显高于阳性对照BHT的EC₅₀值（分别为8.21和6.16 mg/L）。

关键词: 向日葵, 精油, 化学成分, 抗氧化活性

Abstract:

The essential oil of Helianthus annuus head which harvested in autumn was extracted by water-steam distillation, and it's components was characterized by gas chromatography-mass spectrometry(GC-MS). The in vitro antioxidant activities of essential oil were studied by 2, 2-di(4-tert-octylphenyl)-1-picrylhydrazyl(DPPH) and 2, 2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) radical scavenging assay. The GC-MS analysis showed 49 kinds of chemical components(comprising 91.16% of the essential oil) were identified from the essential oil of H. annuus head, and α-pinene(28.22%), limonene(6.55%), α-humulene(4.99%), trans-β-farnesene (3.58%) and camphene (3.39%) were the main ingredients. The essential oil had some in vitro antioxidant activity, and the half effective concentration(EC₅₀) of the essential oil against DPPH and ABTS radical were 0.47 and 0.30 g/L, respectively, which significantly higher than that of BHT(8.21 and 6.16 mg/L).

Key words: Helianthus annuus L., essential oil, chemical composition, antioxidant activity

中图分类号:

TQ35
R284

何昶毅,高潇涵,李泽文,刘玲玲,孙彤彤,陈小强. 向日葵盘精油的化学成分及抗氧化活性[J]. 生物质化学工程, 2020, 54(1): 43-48.

Changyi HE,Xiaohan GAO,Zewen LI,Lingling LIU,Tongtong SUN,Xiaoqiang CHEN. Compositions of the Essential Oil of Helianthus annuus Head and Its Antioxidant Activities[J]. Biomass Chemical Engineering, 2020, 54(1): 43-48.

图/表 3

图1

表1

向日葵盘精油的化学成分"

序号 No.	化合物 compound	保留时间/min retention time	分子式 molecular formula	GC含量/% GC content	保留指数retention index(I_R)
序号 No.	化合物 compound	保留时间/min retention time	分子式 molecular formula	GC含量/% GC content	实测值 measured value	文献值 literature value
1	α-蒎烯α-pinene	6.01	C₁₀H₁₆	28.22	940	939
2	莰烯camphene	6.15	C₁₀H₁₆	3.39	953	953
3	桧烯sabinene	6.43	C₁₀H₁₆	2.33	976	977
4	α-水芹烯α-phellandrene	6.86	C₁₀H₁₆	0.89	1011	1012
5	α-萜品烯α-terpinene	6.99	C₁₀H₁₄	0.95	1021	1021
6	柠檬烯limonene	7.14	C₁₀H₁₆	6.55	1030	1029
7	γ-萜品烯γ-terpinene	7.56	C₁₀H₁₆	0.97	1063	1064
8	芳樟醇linalool	8.01	C₁₀H₁₈O	0.54	1099	1098
9	β-侧柏酮β-thujone	8.17	C₁₀H₁₆O	1.89	1110	1116
10	异罗勒烯allo-ocimene	8.36	C₁₀H₁₆	0.96	1132	1129
11	莰酮camphor	8.57	C₁₀H₁₆O	2.51	1139	1140
12	异龙脑isoborneol	8.81	C₁₀H₁₈O	0.92	1156	1161
13	龙脑borneol	8.98	C₁₀H₁₈O	0.40	1167	1167
14	4-松油醇4-terpineol	9.20	C₁₀H₁₈O	0.71	1183	1180
15	桃金娘烯醇myrtenol	9.40	C₁₀H₁₆O	0.87	1197	1197
16	二氢香芹烯醇iso-dihydrocarveol	9.67	C₁₀H₁₈O	1.77	1215	1215
17	长叶薄荷酮pulegone	9.98	C₁₀H₁₆O	0.41	1236	1236
18	乙酸龙脑酯bornylacetate	10.85	C₁₂H₂₀O₂	0.50	1293	1290
19	δ-榄香烯δ-elemene	11.46	C₁₅H₂₄	0.08	1335	1335
20	丁香酚eugenol	11.77	C₁₀H₁₂O₂	0.90	1356	1353
21	α-古巴烯α-copaene	12.10	C₁₅H₂₄	0.20	1378	1377
22	β-榄香烯β-elemene	12.21	C₁₅H₂₄	0.50	1385	1391
23	异喇叭烯isoledene	12.45	C₁₅H₂₄	1.48	1401	1403
24	β-石竹烯β-caryophyllene	12.76	C₁₅H₂₄	0.17	1423	1420
25	γ-榄香烯γ-elemene	12.89	C₁₅H₂₄	1.44	1432	1432
26	反式-α-香柠檬烯trans-α-bergamotene	12.96	C₁₅H₂₄	0.61	1437	1437
27	反式-β-金合欢烯trans-β-farnesene	13.09	C₁₅H₂₄	3.58	1445	1445
28	α-蛇麻烯α-humulene	13.22	C₁₅H₂₄	4.99	1454	1455
29	别香橙烯allo-aromadendrene	13.39	C₁₅H₂₄	1.11	1466	1463
30	γ-依兰油烯γ-muurolene	13.61	C₁₅H₂₄	0.62	1480	1478
31	大根香叶烯D germacrene D	13.73	C₁₅H₂₄	1.33	1489	1490
32	双环大牻牛儿烯bicyclogermacrene	13.84	C₁₅H₂₄	0.38	1497	1495
33	α-依兰油烯α-muurolene	13.94	C₁₅H₂₄	0.47	1503	1503
34	β-没药烯β-bisabolene	14.03	C₁₅H₂₆	0.45	1509	1510
35	γ-杜松烯γ-cadinene	14.07	C₁₅H₂₄	1.54	1512	1515
36	δ-杜松烯δ-cadinene	14.30	C₁₅H₂₄	0.60	1528	1526
37	α-二去氢菖蒲烯α-calacorene	14.46	C₁₅H₂₀	1.23	1539	1541
38	反式橙花叔醇trans-nerolidol	14.65	C₁₅H₂₆O	0.78	1560	1561
39	桉油烯醇spathulenol	14.75	C₁₅H₂₄O	0.85	1575	1577
40	石竹烯氧化物caryophyllene oxide	14.97	C₁₅H₂₄O	0.15	1588	1586
41	α-雪松醇α-cedrol	15.35	C₁₅H₂₆O	1.70	1602	1605
42	γ-桉叶油醇γ-eudesmol	15.78	C₁₅H₂₆O	1.23	1633	1636
43	γ-荜澄茄醇γ-cadinol	15.85	C₁₅H₂₆O	0.34	1638	1640
44	α-荜澄茄醇α-cadinol	16.01	C₁₅H₂₆O	0.20	1649	1652
45	β-桉叶油醇β-eudesmol	16.30	C₁₅H₂₆O	1.15	1670	1671
46	β-荜澄茄醇β-cadinol	16.51	C₁₅H₂₆O	0.63	1685	1689
47	牻牛儿酮germacrone	16.84	C₁₅H₂₂O	2.84	1709	1707
48	异香橙烯环氧化物 isoaromadendrene epoxide	17.19	C₁₅H₂₄O	2.44	1730	1730
49	反式长叶松香芹醇 trans-longipinocarveol	18.16	C₁₅H₂₄O	2.39	1808	1812
	单萜类化合物monoterpenes hydrocarbons	44.26
	氧化单萜类化合物oxygenated monoterpene	10.92
	倍半萜类化合物sesquiterpenes hydrocarbons	20.78
	氧化倍半萜类化合物oxygenated sesquiterpenes	14.70
	其他类化合物others	0.50
	合计total	91.16

表1

图2

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向日葵盘精油的化学成分及抗氧化活性

Compositions of the Essential Oil of Helianthus annuus Head and Its Antioxidant Activities

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