沉香的化学成分及鉴伪技术研究进展

doi:10.3969/j.issn.1673-5854.2018.04.008

生物质化学工程 ›› 2018, Vol. 52 ›› Issue (4): 45-52.doi: 10.3969/j.issn.1673-5854.2018.04.008

沉香的化学成分及鉴伪技术研究进展

陈虹霞¹, 康秀棠², 尤龙杰², 王成章^1,3

1. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042;
2. 福建省香产品质量检验中心, 福建泉州 362600;
3. 中国林业科学研究院林业新技术研究所, 北京 100091

收稿日期:2017-04-07 出版日期:2018-07-30 发布日期:2018-08-02
通讯作者: 王成章,研究员,博士,博士生导师,主要从事天然产物研究与利用方面的工作;E-mail:wangczlhs@sina.com。 E-mail:wangczlhs@sina.com
作者简介:陈虹霞(1983-),女,浙江舟山人,助理研究员,博士,从事天然产物化学方面的工作;E-mail:shirenyahui@126.com
基金资助:
中国林科院林产化学工业研究所研究团队建设创新工程项目（LHSXKQ5）

Research Progress of Chemical Component and Authenticity Assessment Technology of Agarwood

CHEN Hongxia¹, KANG Xiutang², YOU Longjie², WANG Chengzhang^1,3

1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Fujian Quality Inspection Center of Incense Products, Quanzhou 362600, China;
3. Research Institute of Forestry New Technology, CAF, Beijing 100091, China

Received:2017-04-07 Online:2018-07-30 Published:2018-08-02

摘要/Abstract

摘要： 概述了国内外沉香的主要化学成分，包括挥发油、2-（2-苯乙基）色酮类和三萜类等。介绍了沉香品质检测和鉴伪的现代分析技术，主要有薄层色谱（TLC）、紫外光谱（UV）、气相色谱-质谱联用（GC-MS）、高效液相色谱（HPLC）、X射线衍射光谱（XRD）、傅里叶变换红外光谱（FT-IR）、近红外光谱（NIR）、电子鼻检测和分子生物学技术等。分析比较了各种检测技术的应用范围，指明基于样品特征性谱图库数据建立的多种鉴别技术相结合的方式是未来沉香鉴伪技术发展的趋势。

关键词: 沉香, 化学成分, 掺假, 鉴伪技术

Abstract: The chemical components of agarwood were reviewed, included volatile oil, 2-(2-phenylethyl)chromone and triterpenoids. The modern analytical technologies of the agarwood quality detection and authenticity assessment were described, such as thin-layer chromatography(TLC), ultraviolet spectrum(UV), gas chromatography-mass spectrometry(GC-MS), high performance liquid chromatography(HPLC), X-ray diffraction(XRD), Fourier transform infrared spectroscopy(FT-IR), near infrared spectroscopy(NIR), electronic nose analyzer and molecular biological technique. The applicability and characteristics of various agarwood analytical methods were compared, which provided the reference for the stablishment of quality standard and industry development of agarwood.

Key words: agarwood, chemical component, authenticity, authenticity assessment technology

中图分类号:

TQ35

陈虹霞, 康秀棠, 尤龙杰, 王成章. 沉香的化学成分及鉴伪技术研究进展[J]. 生物质化学工程, 2018, 52(4): 45-52.

CHEN Hongxia, KANG Xiutang, YOU Longjie, WANG Chengzhang. Research Progress of Chemical Component and Authenticity Assessment Technology of Agarwood[J]. bce, 2018, 52(4): 45-52.

参考文献

[1] 国家药典委员会编. 中华人民共和国药典:一部[S].2010年版. 北京:中国医药科技出版社,2010:172-173.
[2] AKTER S,ISLAM M T,ZULKEFELI M,et al. Agarwood production:A multidisciplinary field to be explored in Bangladesh[J]. International Journal of Pharmaceutical and Life Sciences,2013,2(1):22-32.
[3] NAEF R. The volatile and semi-volatile constituents of agarwood, the infected heartwood of Aquilaria species:A review[J]. Flavour & Fragrance Journal,2015,26(2):73-87.
[4] KAKINO M,IZUTA H,ITO T,et al. Agarwood induced laxative effects via acetylcholine receptors on loperamide-induced constipation in mice[J]. Bioscience Biotechnology & Biochemistry,2010,74(8):1550-1555.
[5] YAN Y,WULIJI O,ZHAO X J,et al. Effect of essential oil of Syringa pinnatifolia Hemsl. var. alashanensis on ischemia of myocardium, hypoxia and platelet aggregation[J]. Journal of Ethnopharmacology,2010,131(2):248-255.
[6] 杨峻山. 沉香化学成分的研究概况[J]. 天然产物研究与开发,1998,10(1):99-103.
[7] NOR AZAH M A,CHANG Y S,MAILINA J,et al. Comparison of chemical profiles of selected gaharu oils from Peninsular Malaysia[J]. The Malaysian Journal of Analytical Sciences,2008,12(2):338-340.
[8] SUBASINGHE S M C U P,HETTIARACHCHI D S. Characterisation of agarwood type resin of Gyrinops walla Gaertn growing in selected populations in Sci Lanka[J]. Industrial Crops and Products,2015,69:76-79.
[9] CHEN H Q,WEI J H,YANG J S,et al. Chemical constituents of agarwood originating from the endemic genus Aquilaria plants[J]. Chemistry & Biodiversity,2012,9(2):236-250.
[10] 吴晓鹏,杨锦玲,梅文莉,等. 5批化州产沉香挥发性成分的GC-MS分析[J]. 热带生物学报,2016,7(4):500-509.
[11] 林娜,陈静,张万科,等. 海南产沉香挥发油成分研究[J]. 海南医学,2016,27(9):1383-1385.
[12] 梅文莉,曾艳波,刘俊,等. 五批国产沉香挥发性成分的GC-MS分析[J]. 中药材,2007,30(5):551-555.
[13] TAJUDDIN S N,YUSOFF M M. Chemical composition of volatile oils of Aquilaria malaccensis(Thymelaeaceae) from Malaysia[J]. Natural Product Communications,2010,5(12):1965-1968.
[14] 谷田,彭海刚. 奇楠沉香挥发油化学成分分析[J]. 广东化工,2012,39(4):257-258.
[15] ZHANG Z,HAN X M,WEI J H,et al. Compositions and antifungal activities of essential oils from agarwood of Aquilaria sinensis(Lour.) Gilg induced by Lasiodiplodia theobromae(Pat.) Griffon. & Maubl[J]. Journal of the Brazilian Chemical Society,2014,25(1):20-26.
[16] SHIMADA Y,TOMINAGA T,KONISHI T,et al. Studies on the agarwood(Jinko):I.Structures of 2-(2-phenylethyl) chromone derivatives[J]. Chemical and Pharmaceutical Bulletin,1982,30(10):3791-3795.
[17] SHIMADA Y,KONISHI T,KIYOSAWA S,et al. Studies on the agalwood(Jinko):IV.Structures of 2-(2-phenylethyl) chromone derivatives, agarotetrol and isoagarotetrol[J]. Chemical and Pharmaceutical Bulletin,1986,34(7):2766-2773.
[18] 杨德兰,梅文莉,杨锦玲,等. GC-MS分析4种奇楠沉香中致香的倍半萜和2-(2-苯乙基)色酮类成分[J]. 热带作物学报,2014,35(6):1235-1243.
[19] 杨锦玲,梅文莉,蔡彩虹,等. 黄奇楠生物活性和化学成分的研究[J]. 中成药,2015,37(8):1740-1747.
[20] YAGURA T,ITO M,KIUCHI F,et al. Four new 2-(2-phenylethyl)chromone derivatives from withered wood of Aquilaria sinensis[J]. Chemical Pharmaceutical Bulletin,2003,51(5):560-564.
[21] WU B,KWON S W,HWANG G S,et al. Eight new 2-(2-phenylethyl)chromone (=2-(2-phenylethyl)-4h-1-benzopyran-4-one) derivatives from Aquilaria malaccensis agarwood[J]. Helvetica Chimica Acta,2012,95(9):1657-1665.
[22] SUZUKI A,MIYAKE K,SAITO Y,et al. Phenylethylchromones with in vitro antitumor promoting activity from Aquilaria filaria[J]. Planta Medica,2016,83(3/4):300-305.
[23] 林立东,戚树源. 国产沉香中的三萜成分[J]. 中草药,2000,31(2):89-90.
[24] 刘军民,高幼衡,徐鸿华,等. 沉香化学成分研究(Ⅱ)[J]. 中草药,2007,38(8):1138-1140.
[25] HASHIM Y Z H Y,KERR P G,ABBAS P,et al. Aquilaria spp.(agarwood) as source of health beneficial compounds:A review of traditional use, phytochemistry and pharmacology[J]. Journal of Ethnopharmacology,2016,189:331-360.
[26] AZAH M N,HUSNI S S,MAILINA J,et al. Classification of agarwood(gaharu) by resin content[J]. Journal of Tropical Forest Science,2013,25(2):213-219.
[27] AMIN M R M,BEJO S K,ISMAIL W I W,et al. Colour extraction of agarwood images for fuzzy C-means classification[J]. Walailak Journal of Science and Technology,2012,9(4):445-459.
[28] 李志俊,任婧昱,匡洪尧. 沉香及其伪品的鉴别[J]. 中药材,2011,34(1):59-61.
[29] 黄如栋,陈明,吴家宁. 进口沉香与劣沉香的紫外光谱鉴别[J]. 福建中医学院学报,2001,11(3):47-48.
[30] 李劲松. 沉香X-射线衍射图谱、红外光谱、紫外光谱分析[D]. 海口:海南大学硕士学位论文,2014.
[31] 杨锦玲,梅文莉,董文化,等. 3种越南产沉香的GC-MS分析[J]. 热带作物学报,2015,36(8):1498-1504.
[32] GAO X X,XIE M R,LIU S F,et al. Chromatographic fingerprint analysis of metabolites in natural and artificial agarwood using gas chromatography-mass spectrometry combined with chemometric methods[J]. Journal of Chromatography B,2014,967:264-273.
[33] 吴惠勤,侯思润,黄晓兰,等. SPME/GC-MS鉴别沉香真伪方法研究[J]. 分析测试学报,2016,35(11):1369-1375.
[34] 张倩,霍会霞,顾宇凡,等. 沉香药材HPLC-DAD特征图谱研究[J]. 中国药学杂志,2015,50(3):213-216.
[35] 陈亚. 沉香化学成分和质量评价研究[D]. 广州:广州中医药大学硕士学位论文,2005.
[36] 戚树源,林立东,胡厚才. 白木香中色酮类化合物的形成[J]. 中草药,2000,31(9):658-659.
[37] 何梦玲,戚树源,胡兰娟. 白木香悬浮培养细胞中2-(2-苯乙基)色酮化合物的诱导形成[J]. 广西植物,2007,27(4):627-632.
[38] 李劲松,梅文莉,董文化,等. 国产沉香与白木香X射线衍射指纹图谱比较及相似度分析[J]. 中华中医药杂志,2015(9):3275-3278.
[39] 于玥,张贵君,王晶娟,等. 四种棋楠沉香的FT-IR鉴定[J]. 现代生物医学进展,2010,10(4):746-747.
[40] QU L,CHEN J B,ZHANG G J,et al. Chemical profiling and adulteration screening of Aquilariae lignum Resinatum by Fourier transform infrared(FT-IR) spectroscopy and two-dimensional correlation infrared(2D-IR) spectroscopy[J]. Spectrochimica Acta Part A:Molecular & Biomolecular Spectroscopy,2017,174:177-182.
[41] 钟建理,饶伟文,谢黔峰,等. 沉香的近红外光谱法鉴别初探[J]. 西北药学杂志,2010,25(4):273-275.
[42] NAJIB M S,TAIB M N,ALI N A M,et al. Classification of agarwood grades using ANN[C]//Pahang:International Conference on Electrical, Control and Computer Engineering. New York:IEEE,2011:367-372.
[43] 李志远. 基于电子鼻技术的沉香气味识别及其物质基础研究[D]. 北京:北京中医药大学硕士学位论文,2015.
[44] LEE S Y,NG W L,MOHAMED R. Rapid species identification of highly degraded agarwood products from Aquilaria using real-time PCR[J]. Conservation Genetics Resources,2016,8(4):581-585.
[45] 高晓霞,刘少烽,陈晓东,等. 基于ITS2条形码序列鉴定商品沉香基源植物[J]. 时珍国医国药,2015,26(8):1932-1935.
[46] 徐海星,许沛虎,林世和,等. 中药材白英XRD指纹图谱及相似度分析[J]. 中药材,2012,35(1):42-44.
[47] 王树春,吴云山,贺浪冲,等. 中药材当归的X射线衍射Fourier指纹图谱鉴定[J]. 中药材,2003,26(10):709-711.
[48] 崔晓慧,陈龙,刘义梅,等. 基于X射线衍射指纹图谱鉴别龙骨[J]. 中成药,2016,38(3):624-629.
[49] KUMETA Y,ITO M. Characterization of δ-guaiene synthases from cultured cells of Aquilaria, responsible for the formation of the sesquiterpenes in agarwood[J]. Plant Physiology,2010,154(4):1998-2007.
[50] XU Y H,ZHANG Z,WANG M X,et al. Identification of genes related to agarwood formation:Transcriptome analysis of healthy and wounded tissues of Aquilaria sinensis[J]. BMC Genomics,2013,14(1):227-243.
[51] 钟志敏,吴文如,赖小平,等. 沉香DNA提取方法及种质资源聚类分析研究[J]. 世界科学技术:中医药现代化,2016,18(10):1631-1639.

沉香的化学成分及鉴伪技术研究进展

Research Progress of Chemical Component and Authenticity Assessment Technology of Agarwood

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