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生物质化学工程

生物质化学工程 ›› 2015, Vol. 49 ›› Issue (4): 19-24.doi: 10.3969/j.issn.1673-5854.2015.04.004

• 研究报告 • 上一篇    下一篇

巨尾桉叶中原花色素提取工艺比较及不同溶剂萃取物生物活性的研究

姜萍1, 刘鑫1, 萧伟2, 丁岗2, 赵林果1   

  1. 1. 南京林业大学 化学工程学院, 江苏 南京 210037;
    2. 江苏康缘药业股份有限公司, 江苏 连云港 222001
  • 收稿日期:2015-04-15 出版日期:2015-07-30 发布日期:2015-10-12
  • 通讯作者: 萧伟,教授,博士生导师,主要从事中药新品种、新工艺和新剂型的开发与研究;E-mail:xw@kanion.com。 E-mail:xw@kanion.com
  • 作者简介:姜萍(1969—),女,黑龙江伊春人,副教授,硕士生导师,主要从事天然产物化学与应用;E-mail:pingjiang07@hotmail.com
  • 基金资助:
    广西林产化学与工程协同创新中心基金项目(2013A03);江苏高校优势学科建设工程资助项目(无编号)

Extraction Technology of Proanthocyanidins from Urophylla Leaves and Biological Activities of Different Solvent Extracts

JIANG Ping1, LIU Xin1, XIAO Wei2, DING Gang2, ZHAO Lin-guo1   

  1. 1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
    2. Jiangsu KangYuan Pharmaceutical Co., Ltd., Lianyungang 222001, China
  • Received:2015-04-15 Online:2015-07-30 Published:2015-10-12

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摘要： 通过正交试验探讨了巨尾桉叶中原花色素的3种提取方法的最佳提取工艺条件,并进行了对比。结果显示,传统溶剂提取法的最优工艺为:60%乙醇、80 ℃、100 min、料液比1:14(g:mL,下同),原花色素得率5.95%;微波辅助提取法的最优工艺为:50%乙醇、微波功率200 W、微波时间4 min、料液比为1:20,得率5.48%;超声波辅助提取法的最优工艺为:60%乙醇、60 ℃、超声波时间25 min、料液比为1:14,得率为6.07%。超声波辅助提取法效果最好,时间短,得率高。实验对超声波提取物的不同溶剂萃取物体外抗氧化性和抗肿瘤活性进行了研究,结果表明,乙酸乙酯萃取物抗氧化作用较强,当质量浓度为1.2 g/L时,对DPPH自由基清除率达到96.33%;乙酸乙酯萃取物质量浓度为2 g/L时,对于人肝癌细胞Bel-7404具有很强的抗肿瘤活性,抑制率为56.37%。

关键词: 巨尾桉, 原花色素, 提取工艺, 抗氧化和抗肿瘤

Abstract: The optimum extraction conditions of proanthocyanidins from urophylla leaves using three extraction methods were investigated by using orthogonal experiment.The extracted results were compared.Then,vitro antioxidant and anti-tumor activities of different solvent extracts from ultrasonic extracts were studied.The optimum crafts for traditional solvent extraction method were 60% ethanol,80 ℃,100 min,ratio of material-liquid 1:14(g:mL,the same below),and the obtained yield of proanthocyanidin was 5.95%.The optimum crafts for microwave-assisted extraction were 50% ethanol,microwave power 200 W,4 min for each time,ratio of material-liquid 1:20 with the yield of proanthocyanidin 5.48%.And the optimum crafts for ultrasonic extraction method were 60% ethanol,60 ℃,ultrasonic time 25 min,ratio of material-liquid 1:14,and the yield of proanthocyanidin was 6.07%.Ultrasonic assisted extraction worked best,due to the short extract time and the high yield.The results of antioxidant test showed that the ethyl acetate extracts from ultrasonic extracts exhibited the highest antioxidant activity,as well as the maximum of DPPH radical scavenging ratio was 96.33% with the concentration of 1.2 g/L among different solvent extracts.Experimental results of antitumor activity of different solvent extracts showed that the ethyl acetate extract possessed highest antitumor activity for human hepatoma cell Bel-7404 with the concentration of 2 g/L,and the inhibition rate was 56.37%.

Key words: urophylla, proanthocyanidins, extraction technology, antioxidant and antitumor activity

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