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生物质化学工程 ›› 2013, Vol. 47 ›› Issue (5): 35-39.doi: 10.3969/j.issn.1673-5854.2013.05.007

• 研究报告 • 上一篇    下一篇

杜仲叶渣中杜仲胶提取工艺的研究

谢晓婷1, 路博琼1, 王自波1, 丁来欣2   

  1. 1. 北京林业大学 材料科学与技术学院, 北京 100083;2. 北京林业大学 理学院, 北京 100083
  • 收稿日期:2013-05-14 修回日期:1900-01-01 出版日期:2013-09-30 发布日期:2013-09-30
  • 通讯作者: 丁来欣(1957-),女,江苏南京人,高级实验师,主要从事天然产物化学研究;E-mail:dinglaixin@sina.com。

Extraction Technique of Eucommia Gum from Residue of Eucommia ulmoides Leaves

XIE Xiao-ting1, LU Bo-qiong1, WANG Zi-bo1, DING Lai-xin2   

  1. 1. College of Material and Technology, Beijing Forestry University, Beijing 100083, China;2. College of Science, Beijing Forestry University, Beijing 100083, China
  • Received:2013-05-14 Revised:1900-01-01 Online:2013-09-30 Published:2013-09-30

摘要: 以提取过药用成分的杜仲叶渣为原料,提取杜仲胶。研究了分别用碱水解和酶解预处理杜仲叶渣,然后用溶剂提取杜仲胶,以及直接用溶剂提取杜仲胶的工艺。实验结果表明最佳工艺条件为:杜仲叶渣直接用正己烷作溶剂提取杜仲胶,原料与溶剂比例为1:20(g:mL),机械搅拌并加热回流浸提2h,趁热过滤,滤液放入冰箱在-20℃条件下冷却析晶1h,真空抽滤后自然干燥得到浅绿色杜仲胶产物,提取得率为4.42%。用丙酮多次精制得到白色的杜仲胶。对白色杜仲胶产物做红外光谱测定,确定为反式1,4-聚异戊二烯(TPI)。

关键词: 杜仲叶, 杜仲胶, 提取

Abstract: Residue of Eucommia ulmoides leaves, after being processed for pharmaceutical ingredients, was used to extract Eucommia gum. Both of solvent extraction of Eucommia gum, with pretreatment of E. ulmoides leaf residue by alkali hydrolysis and enzymatic hydrolysis separately, and direct extraction were studied. The optimum process condition was that: E. ulmoides leaf residue was mixed with n-hexane by a material to solvent ratio of 1:20 (g:mL), reflux extraction was applied at a high temperature for 2 h. Filtrate was put in freezer for 1 h at -20 ℃ for cooling crystallization, and then vacuum filtered and naturally dried to obtain the product of light green Eucommia gum. The yield was 4.42%. White Eucommia gum was obtained after multiple times of refining by acetone. The infrared spectrum of white Eucommia gum was measured, It was identified as trans-1, 4-polyisoprene (TPI).

Key words: Eucommia ulmoides leaf, Eucommia gum, extraction

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